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| Registros recuperados: 39 | |
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| Gallay, A; De Valk, H; Cournot, M; Ladeuil, B; Hemery, C; Castor, C; Bon, F; Megraud, F; Le Cann, Pierre; Desenclos, Jc. |
| A large waterborne outbreak of infection that occurred during August 2000 in a local community in France was investigated initially via a rapid survey of visits to local physicians. A retrospective cohort study was then conducted on a random cluster sample of residents. Of 709 residents interviewed, 202 (28.5%) were definite cases (at least three liquid stools/day or vomiting) and 62 (8.7%) were probable cases (less than three liquid stools/day or abdominal pain). Those who had drunk tap water had a three-fold increased risk for illness (95% CI 2.4-4.0). The risk increased with the amount of water consumed (chi-square trend: p < 0.0001). Bacteriological analyses of stools were performed for 35 patients and virological analyses for 24 patients.... |
| Tipo: Text |
Palavras-chave: Waterborne outbreak; Rotavirus; Norovirus; Gastroenteritis; Epidemiology; Campylobacter coli. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1696.pdf |
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| Brito,Wilia Marta Elsner Diederichsen de; Munford,Veridiana; Villaça,André Martins; Caruzo,Thabata Alessandra Ramos; Rácz,Maria-Lucia. |
| Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immunoassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus and one sample was group A / subgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one sample was characterized only by G serotyping/genotyping, and did not react with any P primer used. Two electropherotypes were detected and both were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Diarrhea; Bovine; Genotyping; Serotyping. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200015 |
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| Carneiro,Nadya B.; Diniz-Santos,Daniel R.; Fagundes,Sabrina Q.; Neves,Lívio L.; Reges,Rodrigo M. B.; Lima,Eduardo K. P.; Quadros,Vitor H. de Oliva; Soares,Luis E. de Jesus; Silva,Fernando S. R.; Schneiter,Henrique de Gouvêa; Figueiredo,Ísis; Silva,Luciana R.. |
| Little is known about the epidemiology of severe rotavirus-associated gastroenteritis in Brazil. Given the morbidity associated with this condition and the importance of having detailed knowledge about the impact of rotavirus infection on the epidemiology of acute diarrhea in children, especially those with the most severe diarrheal conditions, we retrospectively reviewed the medical records of all pediatric patients admitted to a tertiary hospital in Salvador, Brazil, due to rotavirus-associated gastroenteritis during one year. It was observed that rotavirus was responsible for 15.6% of the hospitalizations caused by diarrhea and/or vomiting during the period of the study and that 87 of 218 (39.1%) patients seen at the emergency room with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Acute diarrhea; Epidemiology; Children. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600013 |
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| Dubois, Eric. |
| Les rotavirus font partie des principaux virus entériques humains susceptibles d'être rencontrés dans l'environnement. Leur mise en évidence par culture cellulaire dans des prélèvements est longue et peu sensible, ce qui explique le manque de données sur la contamination du littoral. La RT-seminested PCR a été développée pour pallier ces inconvénients. Après une mise au point méthodologique cette technique a été utilisée lors d'études in vitro et in situ. Les études in vitro sur le devenir des rotavirus en eau de mer, suivi par RT-PCR quantitative et par multiplication en culture cellulaire, montrent que dans des conditions proches de celles du milieu naturel (eau de mer non stérile et à une température relativement basse), la détection d'ARN viral peut... |
| Tipo: Text |
Palavras-chave: Rotavirus; Contamination; Coquillages; Eaux de stations d'épuration; Echantillons cliniques; RT-PCR; RFLP; Epidémiologie moléculaire. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/00442/55376/56894.pdf |
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| Langoni,Helio; Linhares,Antonio Carlos; Avila,Fernando Antonio de; Da Silva,Aristeu Vieira; Elias,Acacia Orieth. |
| Two hundred and three samples of feces of calves aged less than 30 days old from both sexes and from different properties and regions of the state of São Paulo were examined in the period of two years. Bacterial cultures were carried out in bovine 10% bloodagar and Levine mediums, incubated for up to 96 hours in aerobic conditions at 37°C, with the observation of colonies and morphological and biochemical study to characterize isolated microorganisms, or other tests, when pertinent. ELISA was performed for the Rotavirus research. The Cryptosporydium spp was surveyed and its parasitological examination was made. Results revealed the involvement of several enteropathogens alone and associated. Rotavirus was found in 51 (25.1%) samples, being 58.8% alone and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Calves; Etiology; Rotavirus; Cryptospodium. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000500004 |
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| Nozawa,Carlos M; Kerntopf,Gustavo Franthesco; Czernisz,Érika da Silva; Albuquerque,Daniele; Romanin,Priscila; Freitas,José Felipe Eliseu; Santos,Norma; Benati,Fabrício José; Pietruchinski,Eduardo; Linhares,Rosa Elisa Carvalho. |
| Acute diarrheal disease is still one of the major public health problems worldwide. Rotaviruses (RV) are the most important viral etiologic agents and children under five years of age are the target population. OBJECTIVE: To investigate the rate of RV infection in hospitalized patients due to acute diarrhea in the cities of Ponta Grossa, Londrina and Assai - Paraná. METHODS: Latex agglutination (LA); immunochromatography (ICG); polyacrylamide gel electrophoresis (PAGE) and negative staining electron microscopy (ME) tests were used to detect the virus. For the genotyping, RT-PCR and RT-PCR-ELISA were used, respectively, for NSP4 and VP4/VP7. RESULT: Out of 124 samples there were 69 positive stool samples for RV, for at least one of the used tests, 67 of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Determination; Hospitalized patients; Genotyping. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600002 |
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| Dulgheroff,Ana Carolina Bernardes; Silva,George Allan Villarouco da; Naveca,Felipe Gomes; Oliveira,Adriana Gonçalves de; Domingues,André Luiz da Silva. |
| ABSTRACT Group A rotaviruses are the main causative agent of infantile gastroenteritis. The segmented nature of the viral genome allows reassortment of genome segments, which can generate genetic variants. In this study, we characterized the diversity of the VP7, VP4 (VP8*), VP6, NSP4, and NSP5 genes of the rotaviruses that circulated from 2005 to 2011 in the Triângulo Mineiro (TM) region of Brazil. Samples with genotypes G2 (sublineages IVa-1 and IVa-3), G1 (sublineage I-A), G9 (lineage III), G12 (lineages II and III), G8 (lineage II), G3 (lineage III), P[4] (sublineages IVa and IVb), P[8] (sublineages P[8]-3.6, P[8]-3.3, and P[8]-3.1), I2 (lineage VII), E2 (lineages VI, XII, and X), and H2 (lineage III) were identified. The associations found in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Infantile diarrhea; Genotype; Genetic variation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300731 |
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| Simaluiza-Masabanda,Rosa Janneth; Cevallos-Solórzano,Gabriela; Bourdett-Stanziola,Lurys; Arévalo-Jaramillo,Paulina; Ruiz-Ramón,Jeimmy; Villacis-Villa,Diana; Torres-Bailón,Paula C; Fernández Jaramillo,Heriberto. |
| Fueron estudiadas, para la búsqueda de rotavirus, 341 muestras fecales obtenidas de sendos niños menores de cinco años con diarrea aguda, atendidos en dos centros de salud de la ciudad de Loja - Ecuador. De ellas, 56 (16%) fueron positivas para rotavirus, siendo genotipificadas 33 muestras para la determinación de genotipos G y P. Los genotipos más frecuentes fueron G4 (42%), P[6] (36%) y la combinación mixta G4+9P[6] (21%). Este es el primer estudio de carácter molecular en rotavirus realizado en el sur de Ecuador y los datos obtenidos ratifican la variabilidad de cepas de rotavirus circulantes en este país. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Genotipos; Ecuador. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572016000400015 |
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| Cardoso,D.D.P.; Rácz,M.L.; Azevedo,M.S.P.; Martins,R.M.B.; Soares,C.M.A.. |
| The G genotyping of 74 group A rotavirus samples was done by RNA-DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4 and the predominance of G1. The knowledge of genotypes provides important information concerning rotavirus circulation in Central Brazil. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Rotavirus; G genotyping; VP7; Oligonucleotides. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400005 |
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| Meneghetti,Ângelo Cesar; Bolognini,Andrea Maria; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Santos,Norma; Nozawa,Carlos Mitihiko. |
| Rotaviruses are common pathogens and the causal agents of acute diarrhea among children and young animals. The involvement of rotavirus in human diarrheal disease among population of urban and rural areas of the city of Londrina, Parana was evaluated. Nine hundred and five fecal specimens from persons with diarrhea were studied, being 686 and 219 from urban and rural areas, respectively. Thirty-eight samples (4,2%) were positive for rotavirus by polyacrylamide gel electrophoresis of viral RNA and latex agglutination test of which 36 were from urban and two from rural areas. Out of the positive specimens, 17 strains were further characterized by RT-PCR typing assay, resulting in 16 strains of G1 genotype while one sample was found to be a mixture of G1 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Diarrhea; PAGE; LA; PCR. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300006 |
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| Guerreiro,AN; Moraes,CCG; Marinho,ANR; Barros,BCV; Bezerra,DAM; Bandeira,RS; Silva,RR; Rocha,DCC; Meneses,AMC; Luz,MA; Paz,GS; Mascarenhas,JDP. |
| ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Animals; Amazônia; Picobirnavirus; Reovirus; Rotavirus; RT-PCR.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000100161 |
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| Serravalle,Karina; Santos,Norma; Sardi,Silvia Inês; Silva,Sarah Peregrino Santos; Ribeiro Junior,Hugo da Costa; Mattos,Ângela Peixoto de; Campos,Gúbio Soares. |
| Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of this study was to characterize the genotypes of Human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA in Salvador, Bahia, Brazil, during the years of 1999, 2000 and 2002. Fecal samples were analyzed (n=358) by methods EIARA and SDS-PAGE for detection of Rotavirus. Positive samples of one or two of these methods (n=168) were submitted to RT-PCR and Multiplex-Nested PCR to determine genotypes G and P. A hundred sixty-eight (46.9%) samples were positive and 190 (53.1%) negative. Only 17 (4.7%) samples had divergent results. The distribution of genotypes G during the first year, showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; PCR; Genotype; Children. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100010 |
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| Registros recuperados: 39 | |
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